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11 Aug 2004, 4:18 AM
| #1 (permalink) |
| i'm sorry for your face.  HSC: 2004 Gender: Female Location: somewhere better than you
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23 Jul 2009, 11:40 AM  | Chromatography You can hide this advertisement by registering. If anyone has any information, any at ALL and any methods of chromatography that I could carry out, please let me know? I have some assignment, I can't remember what it is and I don't have it here with me but I know I have to separate ink or something or other...lol I have no idea.But any information at all that people could contribute about chromatography would be much appreciated 
__________________ B. Arts & Sciences (German & Pharmacology), USYD B. Health Science, USYD M. Health Informatics, USYD B. Science (Behavioural), Swinburne University Hawthorne I'm mean because you're stupid. |
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13 Aug 2004, 3:36 AM
| #2 (permalink) |
| Ancient Orator  HSC: 2004 Gender: Male Location: Penrith
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1 Jan 2009, 6:28 PM   | It is a technique used to seperate substances which have similiar chemical and physical properties. There are different kinds of chromatograpghy such as paper, thin layer, column, gas and high pressure liquid. To seperate ink, use paper chromatograpghy. A piece of absorbant represents the statioanry phase, and the mobile phase is a solvent which flows over the stationary phase to seperate the components of the mixture. So what youd do is put a small dot of the ink on a piece of the absorbant paper, about 2 cm above its edge, then place this end in a container that contains the solvent (but the solvent shouldnt cover the dot of ink when its placed in  ). So then yeah, the solvent be abosrobed up the paper and will seperate the ink, and youll see the different colours that make it up. I know its brief, possibly not even correct, but meh. Last edited by Gavvvvvin; 13 Aug 2004 at 3:40 AM. |
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14 Aug 2004, 2:08 PM
| #3 (permalink) |
| Executive Member  HSC: 2004 Gender: Male
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19 Dec 2006, 12:34 PM | That seems correct as far as I can tell. Paper chromatography works on the different solubilties the different amino acids (when seperating proteins) have in the two phases; mobile and stationary. Stationary phase is polar so the polar amino acids will dissolve in this phase meaning they will travel slowly up the paper. Less polar amino acids dissolve in the mobile phase meaning they travel relatively quickly up the paper. As they say "like dissolves like". Causing the seperation. Obviously this can also be used to seperate the inks in pen inks. Could someone please confirm if this is right.
__________________ "Believe it or not, George, isn't at home, please leave a message at the beep. I must be out or I'd pick up the phone. Where could I be? Believe it or not, I'm not home." "Thanks, Bridesmaid. Like the beard. Gives me something to hang on to" Last edited by Paroissien; 14 Aug 2004 at 2:17 PM. |
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6 Aug 2006, 11:26 PM
| #6 (permalink) |
| Xeno HSC: 2005 Gender: Male
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8 Aug 2006, 12:01 AM | Re: Chromatography Here are my chromatography notes from year 12 IB Chemistry... Chech things if they seem very wrong. Should mainly be trustworthy.
__________________ "Paradox: Emerges somewhere along the Ile des Italiens between two puffs of a cigarette. " - Gustave Flaubert |
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