Bored of Studies - View Single Post - HELP...What is the difference between gene cloning and whole animal cloning???

margaret-lise · 13 Jun 2008, 7:26 PM

thats exactly what i just said...
if you have any questions i suggest you ask me, you dont wanna get information from ammeters

thought id just expand on the process
PCR
1. Denaturation
the sample is heated to 95 degrees for 1 to 5 minutes. hydrogen bonds between DNA strands is broken.
2. Annealing
the mixture is cooled to 55 degrees for about 1 minute. primers bond to the start and end of the sequence to be copied.
3. Elongation
the mixture is heated to 72 degrees for about 1 minute. complementary base pairs are added to the single DNA strands to form a double strand of DNA. the reaction is catalyzed by enzyme polymerase and involves the formation of hydrogen bonds.

i assume the gene cloning is the part your having trouble with?

13 Jun 2008, 7:26 PM	#4 (permalink)
margaret-lise Junior Member HSC: 2008 Gender: Female Join Date: Sep 2007 Posts: 23 Last Activity: 2 Oct 2008, 4:25 PM	Re: HELP...What is the difference between gene cloning and whole animal cloning??? thats exactly what i just said... if you have any questions i suggest you ask me, you dont wanna get information from ammeters thought id just expand on the process PCR 1. Denaturation the sample is heated to 95 degrees for 1 to 5 minutes. hydrogen bonds between DNA strands is broken. 2. Annealing the mixture is cooled to 55 degrees for about 1 minute. primers bond to the start and end of the sequence to be copied. 3. Elongation the mixture is heated to 72 degrees for about 1 minute. complementary base pairs are added to the single DNA strands to form a double strand of DNA. the reaction is catalyzed by enzyme polymerase and involves the formation of hydrogen bonds. i assume the gene cloning is the part your having trouble with?