Help:The effect of substrate concentration(milk) on enzyme(rennin) activity. EXP (1 Viewer)

[Muhammed]

Would be great if someone can help me by tomorrow for this?
Really confused about the method and some materials needed? please help
Would great if someone can post up the METHOD if some one has already done it.

Enzyme is: Rennin
Substrate is: Milk

THANKS!

 

[leesh95]

You dissolve powdered milk in water to make milk with different substrate concentration. One test-tube with substrate 1/2 the normal concentration, another with normal substrate concentration, third with twice the substrate concentration and the fourth with 4 times the normal concentration.

Then you dissolve a junket tablet in water and add it to the milk. Record the time taken for it to clot.

 

[LoveHateSchool]

When I did this expt, we use potato for catalase enzyme, with test tubes with H2O2 and detergent in the bottom, the height of froth representing the differing enzyme activity.

Basically the crux of the expt no matter what method you take, is that enzyme activity will increase until a saturation point is reached where all active sites are occupied. In graphical form, it will slowly curve upwards before flattening off. In HSC bio, Just be remembering the shape of the graphs for the three enzyme expts, I could design my expt in test situations.