Processes used to produce Transgenes (1 Viewer)

[04er]

Do we need to know the stuff about restriction enzymes, DNA ligases, and polymerase chain reaction? And if we do, does the formation of recombinant DNA come before 'microinjection', T plasmids, Biolistics and electroporation? Thanks heaps!

 

[Tommy_Lamp]

I think your going into a little to much detail
If your talking about DNA hybridisation, you jsut need to know the basic process: DNA is extracted from cells. It is heated and the double strands of DNA separate. Restriction enzymes are used to snip the strands into smaller pieces. Then the DNA of two different species is mixed together. As the DNA cools the strands collide into each other and realign to form double stranded DNA.

Im not to sure about the recombinant DNA part of your question, though i dont think so.

 

[04er]

I think your going into a little to much detail
If your talking about DNA hybridisation, you jsut need to know the basic process: DNA is extracted from cells. It is heated and the double strands of DNA separate. Restriction enzymes are used to snip the strands into smaller pieces. Then the DNA of two different species is mixed together. As the DNA cools the strands collide into each other and realign to form double stranded DNA.

Im not to sure about the recombinant DNA part of your question, though i dont think so.

i'm not referring to hybridisation. This is the part on transgenic species...

 

[malkin86]

if you're doing Genetics Code Broken, yes. not so sure for blueprint, though.

 

[Tommy_Lamp]

thats why im confused, maybe your in the wrong topic

 

[Mendo]

Well im pretty sure we only got into recombinant DNA when we did Genetics code broken. I've found in bio the best thing to do is remember everything.