Bio enzyme prac...help (1 Viewer)

[k150764]

i have to conduct an enzyme experiment on their concentration of the substrate on the activtity of a names enzyme catalase...the enzyme is a potato

can anyone check my method and see if i'm doing the right thing??
i'm confused



This is my method for my practical: i left a space in front of hydrogen peroxide because the teacher didn't tell us weather we should use 3% or 6%




Method:

• Place 5 test tubes in the test tube rack and label the test tubes 1-5.
• Pour in the volumes of hydrogen peroxide and distilled water in each test tube with the correct volume of the substrate.

This is shown in the table:
Test Tube
1
2
3
4
5
H2O2 (ml)
0
2.5
5.0
7.5
10
Distilled water (ml)
10
7.5
5.0
2.5
0

• Mix the contents of each test tube.
• Cut the potatoes and crush them to increase their SA for more reaction.
• Place the crushed potatoes in the test tubes with the same amount and mark the initial height of the liquid with a marking pen.
• Measure the height of the test tubes after 5 minutes to see if any change or reaction has occurred.

 

[k150764]

i have to conduct an enzyme experiment on their concentration of the substrate on the activtity of a names enzyme catalase...the enzyme is a potato

can anyone check my method and see if i'm doing the right thing??
i'm confused



This is my method for my practical: i left a space in front of hydrogen peroxide because the teacher didn't tell us weather we should use 3% or 6%




Method:

• Place 5 test tubes in the test tube rack and label the test tubes 1-5.
• Pour in the volumes of hydrogen peroxide and distilled water in each test tube with the correct volume of the substrate.

This is shown in the table:
Test Tube
1
2
3
4
5
H2O2 (ml)
0
2.5
5.0
7.5
10
Distilled water (ml)
10
7.5
5.0
2.5
0

• Mix the contents of each test tube.
• Cut the potatoes and crush them to increase their SA for more reaction.
• Place the crushed potatoes in the test tubes with the same amount and mark the initial height of the liquid with a marking pen.
• Measure the height of the test tubes after 5 minutes to see if any change or reaction has occurred.

the table is kinda stuffed.........it's a table showing how many volumes of hydrogen and distilled water should be in each test tube labelled 1-5

 

[jeff.wong]

Yep that's the same pract I did I just posted it up here... http://community.boredofstudies.org/showthread.php?t=191174