Hi
Im going to perform a prac were I have to test the effect of temp on enzyme activity,
the enzyme is something in the liver and the substrate is Hydrogen Peroxide.
Is this correct, heat a beaker with say 100ml of water to 60 degrees, while heating make sure the testube with the substrate is in the beaker, as soon as the temperature hits 60 degrees place the liver in the substrate.
Do I heat the substrate in water bath, as in place it in a test tube, place the test tube with substrate into the water bath, once the water is at 60 degrees, place the liver in the test tube,
Then get new water, new substrate and repat for different temperature.
Any info will be welcomed. Thanks.
Im going to perform a prac were I have to test the effect of temp on enzyme activity,
the enzyme is something in the liver and the substrate is Hydrogen Peroxide.
Is this correct, heat a beaker with say 100ml of water to 60 degrees, while heating make sure the testube with the substrate is in the beaker, as soon as the temperature hits 60 degrees place the liver in the substrate.
Do I heat the substrate in water bath, as in place it in a test tube, place the test tube with substrate into the water bath, once the water is at 60 degrees, place the liver in the test tube,
Then get new water, new substrate and repat for different temperature.
Any info will be welcomed. Thanks.